A simple, precise, accurate and reproducible spectrophotometric method has been developed for Simultaneous estimation of Paracetamol and Caffeine by employing first order derivative zero crossing method in Distilled water. The first order derivative absorption at 272.9 nm (zero cross point of Caffeine) was used for quantification of Paracetamol and 217.9 nm (zero cross point of Paracetamol) for quantification of Caffeine. The linearity was established over the concentration range of 5-25 μg/ml and 1-10 μg/ml for Paracetamol and Caffeine with correlation coefficient (r2) of 0.9987 and 0.9982 respectively. The mean % recoveries were found to be in the range of 99.52% – 99.88% and 98.39% – 98.66% for Paracetamol and Caffeine, respectively. Inter-day and intra-day studies showed repeatability of the method. The method is successfully applied to pharmaceutical formulation, with no interference from excipients as indicated by the recovery study. All validation parameters were within the acceptable range. The proposed method has been validated as per ICH guideline and successfully applied to the simultaneous estimation of Paracetamol (PCM) and Caffeine (CAF) in their combined Tablet dosage form. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific by no heating and no organic solvents are required. Also use of Distilled water as a solvent makes it Cost effective and very economical method.
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